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KMID : 0903519860290010057
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1986 Volume.29 No. 1 p.57 ~ p.61
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An Extraction of Barley Protein and a Comparison of the Protein Composition of Some Barleys
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Abstract
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Total protein from 4 barley varieties(Olbori, Young San-bori, Sacheon 6, and Suwon 228) was separated into albumin, globulin, hordein and glutelin fractions by the Osborne method and the modified Osborne method in a previous report. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for the protein fractions revealed that there was a little difference in the polypeptide composition of each fraction among four varieties. The comparison of hordeins and hordein-Ⅰ, by polyacrylamide gel electrophoresis at pH 3.0 showed a marked difference among the varieties. Hordein-Ⅰ contained a high level of glutamic acid and proline and low level of lysine. And there was little difference in amino acid composition of hordein-Ⅰs which were extracted from the 4 varieties.
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